<b>Preface.</b> <p><b>Contributors.</b></p> <p><b>1 Measurement of Ligand–G-Protein-coupled Receptor Interactions (</b><i>Katie Leach, Celine Valant, Patrick M. Sexton and Arthur Christopoulos</i>).</p> <p>1.1 Introduction.</p> <p>1.2 Methods and Approaches.</p> <p>References.</p> <p><b>2 Second Messenger Assays for G-Protein-coupled Receptors: cAMP, Ca<sup>2+,</sup> Inositol Phosphates<sup><sub>, ERK1/2</sub> (</sup><i>Karen J. Gregory, Patrick M. Sexton, Arthur Christopoulos and Caroline A. Hick</i>).</b></p> <p><b>2.1 Introduction.</b></p> <p><b>2.2 Methods and Approaches.</b></p> <p><b>2.3 Troubleshooting.</b></p> <p><b>References.</b></p> <p><b>3 Use of the [<sup>35</sup>S]GTP</b>γ<b>S Binding Assay to Determine Ligand Efficacy at G-protein-coupled Receptors (</b><i>Elodie Kara and Philip G. Strange</i>).</p> <p>3.1 Introduction.</p> <p>3.2 Methods and Approaches.</p> <p>3.3 Troubleshooting.</p> <p>Acknowledgements.</p> <p>References.</p> <p><b>4 Quantitative Imaging of Receptor Trafficking</b> (<i>Andy R. James, Takeo Awaji, F. Anne Stephenson and Nicholas A. Hartell</i>).</p> <p>4.1 Introduction.</p> <p>4.2 Methods and Approaches.</p> <p>4.3 Troubleshooting.</p> <p>References.</p> <p><b>5 Production of Recombinant G-Protein-coupled Receptor in Yeast for Structural and Functional Analysis</b> (<i>Richard A.J. Darby, Mohammed Jamshad, Ljuban Grgic, William J. Holmes and Roslyn M. Bill</i>).</p> <p>5.1 Introduction.</p> <p>5.2 Methods and Approaches.</p> <p>5.3 Troubleshooting.</p> <p>References.</p> <p><b>6 Monitoring GPCR–Protein Complexes Using Bioluminescence Resonance Energy Transfer</b> (<i>Werner C. Jaeger, Kevin D.G. Pfleger and Karin A. Eidne</i>).</p> <p>6.1 Introduction.</p> <p>6.2 Methods and Approaches.</p> <p>6.3 Troubleshooting.</p> <p>References.</p> <p><b>7 Using Intramolecular Fluorescence Resonance Energy Transfer to Study Receptor Conformation (</b><i>Cornelius Krasel and Carsten Hoffmann</i>).</p> <p>7.1 Introduction.</p> <p>7.2 Methods and Approaches.</p> <p>7.3 Troubleshooting.</p> <p>References.</p> <p><b>8 A Disulfide Cross-linking Strategy Useful for Studying Ligand-induced Structural Changes in GPCRs</b> (<i>Jian Hua Li, Stuart D.C. Ward, Sung-Jun Han, Fadi F. Hamdan and Jürgen Wess</i>).</p> <p>8.1 Introduction.</p> <p>8.2 Methods and Approaches.</p> <p>8.3 General Considerations, Caveats and Troubleshooting.</p> <p>Acknowledgements.</p> <p>References.</p> <p><b>9 Use of Fluorescence Correlation Spectroscopy to Study the Diffusion of G-protein-coupled Receptors</b> (<i>Stephen J. Briddon, Jonathan A. Hern and Stephen J. Hill</i>).</p> <p>9.1 Introduction.</p> <p>9.2 Methods and Approaches.</p> <p>9.3 Troubleshooting.</p> <p>References.</p> <p><b>10 Identification and Proteomic Analysis of GPCR Phosphorylation</b> (<i>Kok Choi Kong, Sharad C. Mistry and Andrew B. Tobin</i>).</p> <p>10.1 Introduction.</p> <p>10.2 Methods.</p> <p>Acknowledgements.</p> <p>References.</p> <p><b>11 Measurement and Visualization of G-Protein-coupled Receptor Trafficking by Enzyme-linked Immunosorbent Assay and Immunofluorescence</b> (<i>Stuart J. Mundell, Shaista P. Nisar and Eamonn Kelly</i>).</p> <p>11.1 Introduction.</p> <p>11.2 Methods and Approaches.</p> <p>11.3 Troubleshooting.</p> <p>References.</p> <p><b>12 Substituted Cysteine Accessibility Method (SCAM)</b> (<i>George Liapakis and Jonathan A. Javitch</i>).</p> <p>12.1 Introduction.</p> <p>12.2 Methods and Approaches.</p> <p>12.3 Troubleshooting.</p> <p>References.</p> <p><b>13 Homology Modelling of G-Protein-coupled Receptors</b> (<i>John Simms</i>).</p> <p>13.1 Introduction.</p> <p>13.2 Methods and Approaches.</p> <p>13.3 Troubleshooting.</p> <p>13.4 Automated Methods for Generating Models of GPCRs.</p> <p>References.</p> <p><b>Appendix: Site-directed Mutagenesis and Chimeras (</b><i>Alex Conner, Mark Wheatley and David Poyner</i>).</p> <p>A.1 Introduction.</p> <p>A.2 Why Mutagenesis?</p> <p>A.3 Troubleshooting.</p> <p>A.4 Conclusion.</p> <p>References.</p> <p>Index. </p>